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H-L-Sec(MDNPE)*TFA
Nom chimique: (2R)-2-amino-3-((1-(6-nitrobenzo[d][1,3]dioxol-5-yl)ethyl)selanyl)propanoic acid trifluoroacetate // Synonymes: 2235373-47-2 net, PSc, Photo-caged selenocysteine, H-sec(methyl-o-nitropiperonyl), Se-(methyl-o-nitropiperonyl)-selenocysteine,[(R,S)-1-[4',5'-(methylenedioxy)-2'-nitrophenyl]ethyl]-L-selenocysteine trifluoroacetate
- n° Art.:HAA9230
- n° CAS:2235373-48-3
- Formule:C12H14N2O6Se*CF3CO2H
- Masse moléculaire:361,22*114,02 g/mol
- Pureté:min. 98%
- Pureté Énantiomérique:min. 99,7%
Starting at 324,00 €
description
Sec allows the site-specific covalent protein modification, even in the presence of native cysteine, as the reactivity of a selenol group exceeds that of a thiol group. This photocaged selenocysteine (PSc) is incorporated as an unnatural amino acid in response to an amber stop codon using a mutant pyrrolysyl-tRNA/tRNA synthetase pair. Decaging can be achieved by irradiation with UV light (365 nm).
references
Site-Specific Incorporation of Selenocysteine by Genetic Encoding as a Photocaged Unnatural Amino Acid; A. P. Welegedara, L. A. Adams, T. Huber, B. Graham, G. Otting; Bioconjugate Chem. 2018; 29(7): 2257-2264. https://doi.org/10.1021/acs.bioconjchem.8b00254.
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