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Bz-QAR-Rh110-p
Nom chimique: N-Benzoyl-glutaminyl-alanyl-arginyl-Rh110-D-proline // Synonymes: Benzoyl-Gln-Ala-Arg-Rhodamine(110)-D-Pro, substrate for trypsine, prostatin, matriptase
- n° Art.:LS-3530
- Formule:C46H50N10O9
- Masse moléculaire:886,95 g/mol
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description
This asymmetric Rhodamine 110 substrates for trypsine, prostatin, and matriptase employs a simple and straight forward kinetics upon analysis. Absorption wave length 492 nm, emission wave length 529 nm. While the intact substrate is virtually non-fluorescent, fluorescence largely increases (approx.. 3500 fold) upon cleavage. Superior signal-to-noise ratio combined with high sensitivity. In Rh110 assays this substrate delivers much less false positive hits in synthetic product libraries than corresponding AMC (7-amino-4-methylcoumarin) substrates.
references
Chemical Synthesis of Ubiquitin, Ubiquitin-Based Probes, and Diubiquitin; Farid El Oualid, Remco Merkx, Reggy Ekkebus, Dharjath S. Hameed, Judith J. Smit, Annemieke de Jong, Henk Hilkmann, Titia K. Sixma, and Huib Ovaa; Angew. Chem. Int. Ed. 2010; 49: 10149 -10153. DOI: 10.1002/anie.201005995.
C. Wiesmann et al.; J. Mol. Biol. 2012; 419: 4-21.
S.K. Grant et al.; J. Biomol. Screen. 2002; 7(6): 531-540.
U. Hassiepen et al.; Anal. Biochem. 2007; 371: 201-207.
S.P. Leytus et al.; Biochem. J. 1983; 209: 299-307.
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